Hi all, does anyone have recommendations for mRNA amplification kits specifically for adult zebrafish? Currently working with minimal brain tissue samples. Thanks!
There is an error message "interface silicon/oxide not found in grid xxx.tdr file"
26 June 2024 5,551 0 View
Dear all, I want to calculate the phonon dispersion of 4CNR-1-0 (structure is in Fig. 2 (b) in this article Designing coved graphene nanoribbons with charge carrier mob... ) and then calculate...
17 June 2024 6,911 2 View
Monomer: NIPAM; Initiator: BiBB; Catalyst: CuBr; Ligand: PMDETA I added CuBr and the mixture was blue, after that, I purged with nitrogen for 30 minutes and the mixture turned green. The mixture...
27 May 2024 9,144 1 View
CIR 2021/808 written as "the CCα shall be calculated as follows: Method 1: by the calibration curve procedure according to ISO 11843-1:1997 ( 14) (here referred to as critical value of the net...
13 May 2024 9,481 0 View
We bought the E-beam evaporation system without a loading chamber. until now, we have used this system about 20~30 times for the deposition of Al2O3, Ti, Au, and Al having thicknesses from 50~100...
23 April 2024 5,156 4 View
I've recently been trying to investigate the inhibition of H2S-synthesising enzymes: CBS, CSE and 3-MST, via PCR. We've ordered EGCG, PAG and i-3MT-3 to inhibit each enzyme respectively, and have...
16 March 2024 392 1 View
To have multiple case study exploration among Malaysia restaurants that study the impact of servant leadership, what are the best statistical approaches for the case studies?
01 March 2024 2,737 1 View
During the fabrication of the microfluidic chamber (check its structure in the file, a 5x2.4mm microfluidic chamber with height of 20 μm), the ceilings of the chamber often collapse when bonding...
27 February 2024 6,330 3 View
I see in some references like doi: 10.1021/acs.analchem. 1c02335 and /10.1016/j.colsurfb.2022.113114, mention that it is necessary to dilute the concentration of surfactant during the process of...
27 February 2024 9,571 2 View
I want to test out some new primers, but I know from past PCRs that at least one of my reagents is contaminated. I'm on a time crunch to finish my project and am not sure if I can get new reagents...
21 February 2024 4,799 0 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
Information in The Three Dimensions of Time.Information in The Three Dimensions of Time Information Transfer based on Brains Entanglement.Information Transfer based on Brains Entanglement Time...
28 July 2024 7,522 0 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View