BrdU staining issue?

Hi everyone. I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain...

13 January 2021 9,449 0 View

Effective number of species and Wilcoxon Signed Rank Test for diversity between 2 ecosystems: is this accurate?

Hi! So I'm currently studying the diversity difference between 2 ecosystems. I ran a Shannon-Wiener test on site 1 and site 2 and found the indices of 1.525 and 2.165 , respectively. I have done...

22 November 2020 6,798 1 View

How to reproduce the sub-grade of pavements without using soil?

Dear colleagues, In order to evaluate the performance of concrete slabs-on-grade, the sub-grade should also be considered in the testing setup. Using soil or sand to reproduce the sub-grade is a...

20 November 2020 7,921 1 View

How to determination of available silicon in fertilizer by UV-Vis?

Hello everyone! I have a problem with when the determination of available silicon in fertilizer by UV-Vis. I do that following method A 5-Day Method for Determination of Soluble Silicon...

10 November 2020 1,370 10 View

What is the problem with my 10um Nissl stained cell?

Please see the attachment. In my case, I tried a lot of time on the Nissl staining on 10um cryosection. I am not sure which step goes wrong? I make sure my anti-rolling glass is intact. seem like...

07 November 2020 9,568 3 View

Where and how do I specify frequency in the route section of frequency-dependent calculation (Gaussian using Linux)?

Hello everyone. I would like to compute the dynamic hyperpolarizability value of a molecule using Gaussian on Linux OS. I have read that the essential keywords to include for such request are the...

07 November 2020 5,620 4 View

What type of regression should I be using?

I am planning to conduct a study with the following variables: A - independent variable B - intervening variable C - dependent variable D - moderating variable between B and C The framework...

22 October 2020 5,324 3 View

Not sure what does 3mg/kg mean in AD STZ injection?

What I searched the paper online they mentioned do 3mg/kg ICV on mice On Day 1 and Day3. Doese it mean total 3mg/kg for 2 days, or 1.5mg/kg for day1 and another 1.5mg/kg for day3? Help help to...

19 October 2020 8,687 3 View

How do I calculate theoretical fluorescence spectra (TDDFT) in Gaussian 09W in selected solvent?

Good day everyone. I am new to computational chemistry and would like to integrate some part of it in my fluorescence study. I would like to investigate the theoretical fluorescence spectra using...

18 September 2020 6,742 3 View

How to separate peak in HPLC?

13 August 2020 2,331 8 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View