Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View

Has anyone tried performing a transient transfection on a cell line that has been stably infected with a lentivirus?

Hello, I was having difficulty in infecting a cell with a gene of interest. Say, for instance, I was able to introduce a CMV on the cell, but I was not successful in introducing my target gene. I...

25 September 2019 2,412 3 View

Can 2 way ANOVA be used in nonexperimantel research?

We will be studying about the role of spirituality and gender ( 2 IVs) on coping effectiveness (DV) and we shall be using standardized scales in measuring. Can 2 way ANOVA be used in our analysis?...

09 November 2017 8,917 4 View

Is it possible to store LN-2 frozen tissue biopsy samples at -20C?

I am looking to treat thyroid tissue with Iodine-131 and need to hold the tissue in a 'contaminated tissue' freezer, although the freezer is -20C as opposed to -80C. I usually store all tissue at...

06 September 2017 8,124 4 View

Any information or studies about Wild Sampaguita (Jasminum bifarium) ?

My study for my undergraduate thesis is Phytochemical Evaluation, Antibacterial Activity and Angiogenic Property of Wild Sampaguita (Jasminum bifarium). I am using leaves for my study and I need...

25 March 2017 3,487 3 View

Does an electron emit EM radiation when it accelerates or when its energy changes?

Motion in a straight line (non-relativistic) is F = ma, the force changes the velocity and therefore increases the energy E = 1/2mv2. For motion in a circle, the centrifugal (centripetal if you...

05 April 2016 4,493 6 View

Are there any researchers looking at non-wave theories, in particular particle descriptions of quantum effects?

I am particularly interested in liaising with people that are looking at quantum effects by looking at particle (say photon) interactions only, without resorting to wave behaviour. For example,...

17 March 2016 6,884 69 View

What is the best arabidopsis thaliana root RNA extraction method?

I am growing A. thaliana roots on agar plates and trying to extract RNA to use for gene expression analysis. Currently, I can't seem to get a concentration above about 15 ng/ul, and even then,...

03 February 2016 4,772 3 View

Does anyone have troubleshooting experience for low on-target exome enrichment (Nextera)?

I have prepared 4 libraries (Nextera exome 37mb), first with 12 samples and prepared 3 months before the next 3 (of 24 samples). The first had low enrichment (40% of reads on-target), the second...

30 June 2015 3,324 1 View

How common is five-fold symmetry among plants and animals?

06 July 2014 6,460 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View