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Questions related from Mae Riley
Is there a site which determines the size of cDNAs? If someone wants to insert a gene with a fluor tag such as GFP, how would they find out the size of the cDNA for that gene + GFP?
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Does anyone have STR profiles of mouse cell lines from the following strains: C57BL/6 129 FVBN BALB/c Swiss black Which company did you use to authenticate the cells?
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1. Rat primary cortical astrocytes incubated in BCECF 30mins. 2. 20mM NH4-prepulse 5mins. 3. Na-free HEPES 5 mins.
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If I have a 40% chimeric male mouse, how many matings would be needed to get germline transmission to F1? Is there a breeding calculator which provides this information?
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