How to handle HIV viral RNA to isolate it properly for further sequencing?

13 March 2024 0 10K Report

I've been using the Qiagen Circulating extraction kit to extract the viral RNA from plasma samples. Further I used an in-house RT step (very similar to SuperScript IV from invitrogen) to get cDNA. Now my problem is that my cDNA is quantitatively as well as qualitatively very poor. Does anyone have suggestion on how to optimize cDNA yields?

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