Hi there,

Yet another IF-related question.  I'm going to be dual-labelling free-floating tissue sections with fluorescent secondary abs.  After the final wash steps, I will be mounting sections onto slides using a DAPI medium.  

Maybe a silly question but will I have to mount my sections in the dark, or with very minimal light at least so the fluorescence isn't affected?

Or does anyone know if I could mount sections in a dark room with a dark room lamp (does that affect fluorescence?), usually where western blots are developed?

Thanks in advance

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