Hi there,
Yet another IF-related question. I'm going to be dual-labelling free-floating tissue sections with fluorescent secondary abs. After the final wash steps, I will be mounting sections onto slides using a DAPI medium.
Maybe a silly question but will I have to mount my sections in the dark, or with very minimal light at least so the fluorescence isn't affected?
Or does anyone know if I could mount sections in a dark room with a dark room lamp (does that affect fluorescence?), usually where western blots are developed?
Thanks in advance