I am producing monoclonal antibody in HEK293 cells and purifying the supernatant using G resin. the purification involves adding 0.1M Glycine pH= 3 and centrifugation for 1 minute. i do this step more than 12 times. doing gel electrophoresis and western blot confirms that the majority of my antibody is still attached to the resin. is there a simple method that i can elute all of my antibody from the resin?
Bruce Allen
Hello Hisham. There are a few paragraphs in the following article that may help you.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2958570/
0 votes
0 thanks
- Badges
- Science method
More Hisham Alharbi's questions See All
Similar questions and discussions