Monarch® RNA Priming Buffer - any substitutes or Homemade Recipes available?

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At the last of elementary school, a proficient reader is expected to typically read at a rate of about 150 to 200 words per minute .True?

I try to find scientif paper for the speed of reading in last grade if elementary school or general in elementary school. Can soemone help me?

17 April 2024 4,730 3 View

What is the scientific basis for the consumer basket?

Dear colleagues, I am conducting a research study on consumer basket formation, with a focus on the scientific justification behind it. Your expertise in this field would be invaluable for my...

01 April 2024 1,985 1 View

What are the differences in the consumer baskets seen between different countries?

Dear users, Our team is conducting a study on consumer baskets in different countries. We would appreciate your help in providing information about typical products, other expenses, and other...

06 March 2024 4,396 1 View

Why does inlet boundary need to be located far from the body In CFD simulation?

Usually the distance between inlet boundary and body about 5 size of body. For what purpose? Why cannot located body very close to boundary? I simulate the flow around bridge section, VIV and...

04 March 2024 8,439 3 View

Has anyone used ficoll to isolate microglia from whole brain samples?

Could ficoll be used as a substitute for percoll in dencity gradients when performing microglial isolation? Right now, we are trying to isolate microglia for flow cytometry. We are going to use...

24 January 2024 1,534 0 View

How to analyze sequencing data in a very large fastq file?

Hello, I'm trying to figure out how to analyze these fastq files. We received 2 files, one labeled R1 and the other R2. Its raw data and there seems to be some barcodes within the file as well....

25 October 2023 3,624 3 View

What is the accuracy of the data from literature versus the results of Thermal Conductivity Scan (TCS) in determining thermal conductivity of rocks?

Would TCS be superfluous if the literature values are on the safer side? How does it compare in the case of sound rock? Weathered rock?

13 August 2023 4,606 1 View

What is the difference between structured and unstructured mesh for Karman vortex street? or for vortex shedding ?

II'm doing a transient flow calculation around a bridge and I need to get the vortex street and calculate the Strouhal number. To do this, I made a structured mesh. At the beginning of the...

01 June 2023 5,854 8 View

How does discrimination and stigma towards the LGBT community impact their access to healthcare services and their overall health outcomes?

What changes are needed to protect the LGBT community from disparities in medical facilities

18 February 2023 5,549 1 View

How do I serially dilute the unknown protein for the Western Blot Bradford Protocol?

This is my first time trying Western Blotting and I need help with managing the unknown protein quantification using a plate reader (SpectraMax/Softmax Pro software). I have an African cichlid...

22 January 2023 8,829 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View