How do you measure the affinity of a secondary antibody to a primary antibody?

I have conjugated a dye to a goat anti-rabbit secondary antibody. I believe that the more dyes that I conjugate to the antibody, it then loses its ability to recognize the primary antibody. I...

18 June 2020 4,691 3 View

How to identify and retrieve many (+30) RNASeq datasets (Fasta) under a specific criteria in a practical way?

22 March 2020 3,649 3 View

What can be used to simulate stomach contents to be examined in a forensic science teaching lab?

I am always looking for new ways to study subjects when I am teaching Forensic Science. At the moment I am only using a mixture of baby food for my simulated stomach contents. What can I do to...

08 April 2019 9,865 2 View

Identifying clay/zeolite mineral XRD patterns using Match!3?

Hi friends, My university recently switched from using the ICDD Powder Diffraction PDF-4+ database for identifying minerals by XRD to a program called Match!3. For identifying bulk samples,...

26 September 2018 2,537 2 View

Can a DNA profile be obtained from DNA from a fingerprint?

I have read reports from several years past stating that DNA can be obtained from a latent fingerprint. I have not found any recent work on the subject. Do people think it is still feasible and...

31 May 2018 1,016 3 View

How can a cDNA library be contaminated with intronic DNA?

I isolated clones from a preconstructed cDNA library. After sequencing the clones, I found that they did not match known genes via BLAST as they should have, and that the sequence is...

23 April 2018 9,663 5 View

Cultivating Legionella in liquid medium

21 May 2017 3,942 9 View

Does any one know the command string used in paup for CI and RI values?

Does any one know the command string used in paup for CI and RI values? Using Cipres and need to add the command string to get CI and RI values.

28 February 2017 6,515 2 View

Has anyone used R to determine classes based on Latent Trajectory Analysis?

I see there is the lcmm (latent classes mixed models) package for R. What issues did you encounter?

22 February 2017 1,001 1 View

Has anyone used a microplate reader to measure MMP with JC-1?

I have several patient-derived cell lines that are slow-growing and material is limited, so instead of flow cytometry I wanted to try measuring MMP using a fluorescent microplate reader on 96-well...

19 June 2016 6,444 1 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

Rearranging abbreviation list in LaTeX ?

Hi, I'm trying to rearrange an abbreviation list for a manuscript such that it would be listed in alphabetical order. Is there a function that does this automatically or is manual my only option?...

18 February 2021 4,870 2 View

If a journal doesn't provide doi after acceptance of the manuscript, is it authentic?

It is related to submission of the manuscript in a Journal and the process of its online availability.

18 February 2021 9,269 5 View

Can anyone provide an advice on running a validation study of a commercially available product in collaboration with the manufacturer?

We are planning to run a study validating a commercially available product (i.e. software) and while we initially planned to do it independently of the manufacturer, they heard about our intention...

17 February 2021 2,295 3 View

How to increase DPI in my image?

I'm submitting manuscript to a journal that requires 1200 dpi in my images. I have changed the dpi from 96 but when I upload the image with 1200 dpi, the image looks super blurry when I review my...

16 February 2021 2,803 3 View

Can anyone pls provide me information regarding the libraries where from i can get the absorption data of different materials used in solar cells??

I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...

10 February 2021 6,034 7 View

How would I get a "certificate of English approve" ?

I need a "certificate of English approve" for my manuscript to be published in a reputed journal. It should be from a native speaker.

04 February 2021 1,331 3 View

How to perform analysis on Chandrayaan-2 DFSAR data using MATLAB or other languages like python?

Kindly suggest any libraries/Books/platforms/tutorials for the same.

04 February 2021 1,708 2 View

How to remove unedited version of the manuscripts from research gate?

Our manuscript was published ahead of print. The edited and revised version is now available. How do I remove the old version of the manuscript from the research gate?

01 February 2021 5,965 1 View

How is the variance for an image exactly computed to test the uniformness of its histogram?

I am trying to compute the variance of a plain and cipher grayscale LENA image... I found in a paper that variance value is equal to 630780 for a plain Lena image while if I calculate I find it...

28 January 2021 8,036 5 View