How do you measure the affinity of a secondary antibody to a primary antibody?

I have conjugated a dye to a goat anti-rabbit secondary antibody. I believe that the more dyes that I conjugate to the antibody, it then loses its ability to recognize the primary antibody. I...

18 June 2020 4,691 3 View

How to identify and retrieve many (+30) RNASeq datasets (Fasta) under a specific criteria in a practical way?

22 March 2020 3,649 3 View

What can be used to simulate stomach contents to be examined in a forensic science teaching lab?

I am always looking for new ways to study subjects when I am teaching Forensic Science. At the moment I am only using a mixture of baby food for my simulated stomach contents. What can I do to...

08 April 2019 9,865 2 View

Identifying clay/zeolite mineral XRD patterns using Match!3?

Hi friends, My university recently switched from using the ICDD Powder Diffraction PDF-4+ database for identifying minerals by XRD to a program called Match!3. For identifying bulk samples,...

26 September 2018 2,537 2 View

Can a DNA profile be obtained from DNA from a fingerprint?

I have read reports from several years past stating that DNA can be obtained from a latent fingerprint. I have not found any recent work on the subject. Do people think it is still feasible and...

31 May 2018 1,016 3 View

How can a cDNA library be contaminated with intronic DNA?

I isolated clones from a preconstructed cDNA library. After sequencing the clones, I found that they did not match known genes via BLAST as they should have, and that the sequence is...

23 April 2018 9,663 5 View

Cultivating Legionella in liquid medium

21 May 2017 3,942 9 View

Does any one know the command string used in paup for CI and RI values?

Does any one know the command string used in paup for CI and RI values? Using Cipres and need to add the command string to get CI and RI values.

28 February 2017 6,515 2 View

Has anyone used R to determine classes based on Latent Trajectory Analysis?

I see there is the lcmm (latent classes mixed models) package for R. What issues did you encounter?

22 February 2017 1,001 1 View

Has anyone used a microplate reader to measure MMP with JC-1?

I have several patient-derived cell lines that are slow-growing and material is limited, so instead of flow cytometry I wanted to try measuring MMP using a fluorescent microplate reader on 96-well...

19 June 2016 6,444 1 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

Separated objects vs Non-Manifold Assembly?

I'm trying to do a simulation between the Maxilla and the Mandible I converted the Dicom file from the CT scan to a CAD module and module the articular discs via 3d software. I have two options...

08 February 2021 8,731 2 View

How do I apply continuity boundary condition at the edge using form assembly in comsol?

The continuity boundary condition is automatically created for a shared (common) surface boundary but I want to have it for an edge. Please help me out. In image 8 is the base of the cylinder and...

05 February 2021 3,459 2 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

Could someone please suggest a suitable program for assembling plasmids containing repetitive sequences from short paired-end reads?

Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...

03 February 2021 6,379 3 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

Gibson PCR and reaction challenges, any tips?

Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...

29 January 2021 1,406 2 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View

How to Import .inp file from abaqus CAE to Ansys perfectly?

I am trying to import an Abaqus .inp file to Ansys but it does not import the whole assembly but only imports a single part. How to solve this.

17 January 2021 5,450 3 View

What are best free Molecular Dynamics software packages to generate pre-built biological bilayer membranes?

Our task is to numerically invetigate cellular bilayer membrane. In order to numerically simulate behavior of biological complex membrane, we need to have initially constructed membrane. However,...

13 January 2021 7,809 4 View