Mitotic index calculation protocol?

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I need MD simulation in 500 ns for some of my test compounds with PBSA, GBSA and PCA. If a researcher will help, we will provide authorship?

For my research, I need MD simulation of 500 ns with PCA. Please reply if you have facility. We will provide corresponding authorship. The article will be published in a high IF journal. My email-...

26 November 2023 6,926 0 View

How to increase the solubility of chlorophyll extracted from plants for bioimaging studies?

Please provide information related to any coating that can be done or any composites that can be synthesized.

09 October 2023 9,003 2 View

Which families of freshwater fishes belong to 'True hill stream' fish families?

In the freshwater fish groups, some of the genera like Garra, Balitora, Labeo, Scizothorax etc are considered as true hill stream forms. On what basis this classification is considered?

02 August 2023 6,389 0 View

As we all know that Si 2p peak can be deconvoluted into two peaks such as Si 2p1/2 and Si 2p3/2. Which peak has higher binding energy and why ? ?

In the few literature, it was mentioned that the binding energy of Si 2p1/2 was higher than Si 2p3/2, while some authors made the just opposite statement about these peaks. I am so confused about...

22 June 2023 1,135 2 View

What is the JCPDS No and CCDC no for Ag-MOF-5?

Ag-MOF-5 synthesized from Terepthalic acid, Silver Nitrate and DMF. Need to know Chemical formula, Molecular weight and molecular structure of Ag-MOF-5. Thank you

21 May 2023 1,624 0 View

How to normalize total protein in Ponceau S stain?

I used to normalize my target protein using housekeeping genes. But now I have come to know about total protein normalization. So I would like to do the same. I want to know how to measure total...

11 March 2023 9,482 2 View

Can a composite show different dual phases (space group) in room temperature XRD and room temperature Neutron diffraction Reitveld refinement?

If possible kindly suggest some references.

05 February 2023 3,683 3 View

How to isolate fibroblast cells from hamster?

I isolated fibroblast cells from hamster by collagenase A digestion method. I used DMEM with extra L-glutamine as culture media. After 24hr there is no attachment of cells. The flask was coated...

28 November 2022 4,816 1 View

Which is the best technique- FACS or MACS for separation of dead cell from live cell population by using PI and SYTO9 fluorescence dye?

I want to separate dead cells from a bacterial population after treatment of any drug or antimicrobial agent. Which technique will be more efficient FACS or MACS if I use PI and SYTO9 dye ? Is...

15 August 2022 4,009 3 View

How to separate my metal microcage synthesised in ethylene glycol and water solvent ??

I tried to pellet it down at a maximum of 18K rpm, but it did not work. I heated the reaction mixture up to 200 C temp to evaporate the Ethylene Glycol(Including water) and to get the metal...

21 June 2022 6,740 0 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View