Mitochondrial Number: What is the best way to quantify mito number from frozen tissue?

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Nicolas Pichaud

For mitochondrial number, the best proxys are citrate synthase and cardiolipin content... On the other hand, mitochondrial Dna copy is actually a poor marker of mitochondrial density (see Larsen et al. 2012:http://onlinelibrary.wiley.com/doi/10.1113/jphysiol.2012.230185/full).

Citrate synthase is actually the simplest to measure...

Jason Collett

Thank you very much!

Aarti Darra

As said, citrate synthase is a good proxy for quantifying mitochondrial number. But as this enzyme is also found in peroxisomes, how reliable it will be AS A PROXY?