My research project is centered around finding possible PET degrading bacteria strains. In order to test for possible PET degration I need PET film deposited onto coverslips. This way I can coat these coverslips using NileRed for further examining, using the ZOE Fluorescent Cell Imager.
Is there a way to fixate thin PET film onto coverslips without creating undesired speace in between the film and coverslip which could possibly hinder the micrscopic examination?