In Gaussian, how to calculate energy of a low-spin antiferromagnetically coupled binuclear complex?

Dear colleagues, I would like to ask you for help. I have been trying to calculate the energy of a binuclear Cr(II) organometallic complex, where the Cr(II) atoms are connected via hydride...

08 November 2020 563 2 View

Should I test for HWE in dataset of inbred animals without random mating?

Hi, I am doing a GWAS analysis of european bison's a dog's SNPs. Right now I am doing multiple data clean up, according to previous studies and what I have learned. I was thinking, if it is ok to...

18 March 2020 3,103 3 View

Does anybody know an sientific/empirically paper about psychological requirements in teamsports (e.g. ballsports)?

I am writing my Bachelor thesis about psychological requirements in volleyball. I appreciate any help I could get :) Thank you so much

08 February 2020 5,728 4 View

How to compare the results of variation partitioning for two diferent communities?

12 September 2019 6,578 1 View

How actual physical thickness (AOT) of Si02 can be calculated from effective oxide thickness (EOT) ?

As we all know if the EOT of a device is 3nm that doesn't mean that the actual oxide thickness is 3nm. so my question is how we can calculate the Actual oxide thickness (Physical ) of SiO2 from a...

28 July 2019 3,996 1 View

How can the efficiency of soft annealing on aluminium conductor be controled?

How can the wire producer or the customer control the effectivnes of annealing process to get enough soft wire? Are there some limit values of mechanical properties to test Al-conductor class2...

11 December 2018 4,824 5 View

What statistical test to use to find out what taxa significantly differ between several treatments?

20 December 2016 6,444 4 View

No title

when is the counterstaining with haematoxylin good?

31 October 2016 5,499 3 View

V5 His tag N terminal mammalian expression?

Hi, I`m looking for a plasmid to subclone into a mammalian system. It should have V5 and a His tag on N-terminus. Any suggestions?

14 September 2016 2,576 5 View

No title

29 October 2014 8,947 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View