Interesting question. Following the discussion.

Hello,

Apologies - my reply comes rather late, but I hope it might still be useful to you.

Unfortunately, in our own R&D team, we do not have any experiences with this specific cell type, but maybe our Application Note 21 "Wound Healing and Migration" can help to set up the experiment: https://ibidi.com/img/cms/support/AN/AN21_Wound_Healing_Assay.pdf

Here are some publications, in which our Culture-Inserts and HTC-116 cells were used:

https://onlinelibrary.wiley.com/doi/full/10.1002/path.5453 (open access)

https://www.sciencedirect.com/science/article/pii/S2352396419306140 (open access)

https://www.nature.com/articles/s41416-020-0758-1

https://onlinelibrary.wiley.com/doi/abs/10.1111/pin.12811

https://www.sciencedirect.com/science/article/abs/pii/S0304383520300847

If you need more advice or would like to discuss your experiment in more detail, our tech support team ([email protected]) is happy to help.