How can I measure cholesterol level in tissue?

Hi Dear friend, Along as I am working on an animal model study as C57lb/6 mice to determining the cholesterol concentration in blood serum and in the brain But now I am planning to extract the...

18 July 2020 2,998 4 View

Architecture Workshop for 1st stage architectural student?

In the first architectural education, is it possible to add an engineering workshop that includes works other than architectural drawing and computer display?

01 March 2020 9,610 9 View

Can I use the frozen bone to make a histological sections?

If I have frozen the tissue, can I use it to make histological sections? what about the bone tissue ?

16 August 2019 8,916 6 View

In your opinion .... What are the most important challenges to maintain the centers of cities?

Is it possible to preserve the heritage, religious and cultural identity of city centers, and are there standards for conservation that are consistent with modern technology?

01 January 1970 1,284 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

19 February 2021 8,351 2 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

In your view, which current SEM is most recommended for EBSD/TKD and EDS analysis?

I know that this is a very difficult question, as the areas of application can vary greatly. However, if you want to operate low as well as high resolution (from a few millimeters to nanometers),...

02 February 2021 4,985 4 View

Gain and offset values in Sentinel-2 L1C?

Hello everyone. I have been trying to apply radiometric calibration on Sentinel-2 L1C images (bands 2,3,4 and 8), but every time after layerstacking I come across this error: "Calibration...

28 January 2021 7,160 1 View

How can you confirm new liquid FTIR cell fits calibration?

We have an FTIR instrument which was calibrated in transmission/absorption mode using a set of cells for different ranges of D2O concentration. One of the cells at high range broke and the new one...

16 January 2021 4,791 2 View