Hello,
how can I calibrate a microscope just by the stage micrometer slide?
If your microscope has an ocular micrometer (ruler on ocular lens), I suggest you use a stage micrometer as standard (reference). You can compare the value of the stage micrometer image (ruler image on slide) as standard to ocular micrometer as a unit under test. And please consider magnification.
And if your microscope does not have an ocular micrometer, it can not be called "calibration"'. Maybe you need to perform "verification" only on your microscope.
#Hopefully it can answer your question^^
Thank you very much @Okasatria Novyanto ; but what's the role of stage micrometer alone ??? The calibration must be performed by two rulers !!!!
Djouadi Anfal, the role of stage micrometer is standard (reference). For your information that according to ISO / IEC Guide 17025 and Vocabulary of International Metrology (VIM), in simple understanding, Calibration is an activity to determine the conventional truth of the indicated value of measuring instruments by comparing it against traceable measurement standards to national and international standards for units of measure.
Okasatria Novyanto, I know that but how can I calibrate it using just a stage micrometer ??
Practically, maybe the video at the following link can help you.
https://www.youtube.com/watch?v=HaqgCtA-ioI&list=PLvmVX4M4VjNQOoCteOWAP5f18vfFwzFJI&index=12
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