14 December 2011 1 5K Report

Which is the best target prediction tool for miRNA in Chicken?

More Muhasin Asaf's questions See All
What services do you recommend for T-cell repertoire sequencing using RNA from non-lymphoid tissue?

I am wondering if someone has experience they can share (also privately if preferable) about different kits/methods to sequence the T-cell repertoire from RNA derived from non-lymphoid tissue...

27 December 2018 6,099 2 View

Why do I get an error when calculating PBC in Gaussian?

I'm trying to run a PBC calculation of a 16-atom unit cell. My input is: #p opt pbepbe/6-31g(d,p) Int(Grid=Ultrafine) pop=regular iop(5/33=1) I run it on 16 processors and 30GB memory usage. The...

26 December 2018 6,581 0 View

The COD is increased in the experiment rather then decreasing?

hello sir, during my experiment the COD of the paper and pulp mill effluent (black liquor) is increased rather then decreasing after treatment with bacterial isolates. pls help me out

16 December 2018 455 1 View

Simulation based learning

 Hi everyone  I hope all is  well. I I am studying simulation. I would appreciate if any one could tell me what is the difference between simulation based strategies versus simulation technology....

15 August 2017 8,744 6 View

Discrepancies between deep sequencing and qRT-PCR results

Can anybody please troubleshoot why my results based on differential expression of deep sequencing and qRT-PCR are not tallying. A list of miRNAs were found to be upregulated by deep sequencing...

08 May 2012 448 5 View

Ties with Govt ( local or central ) Internal org crisis can anyone recommend Studies

i start my PHD recently i am interesting in to Study Internal organisation Crisis why organisation keep ties with GOvt and what the impact. and CEOs empathy in different stage of org Crisis...

01 January 1970 2,386 0 View

Similar questions and discussions
Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

What is the best way to synthesize cDNA of miRNA; using poly A kit or stem loop PCR?

Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...

16 June 2024 9,723 2 View

Extraction RNA with miRVANA kit?

Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...

06 June 2024 5,715 4 View

I need guidance how to design primers for miRNA?

The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...

23 May 2024 7,570 3 View

Should the target miRNA level decrease after the miRNA inhibitor transfection when checked with qPCR??

I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...

20 May 2024 5,920 4 View

Webserver for identifying activation and repression of TFs, genes, and miRNAs?

Hello. Is there any webserver where I can input a set of transcription factors, genes, and miRNAs, and it can provide me with statistical data on how TFs either activate or repress genes and...

18 May 2024 5,848 1 View

Does anybody face problems with total RNA isolated after miRNA mimic and inhibitor transfection?

Hello all. I have recently been trying to isolate total RNA after transfection of miRNA mimics and antisense inhibitors in mammalian cells. However, the RNA isolated through the trizol method...

13 May 2024 4,935 7 View

I would like to work on microRNA detect colorectal cancer. Which type of microRNA type micRNA124,micro143 how I can make primer design for it ?

would like to work on microRNA to detect colorectal cancer. I will use the type of microRNA type micRNA124,micro143 how I can make primer design for it and how I can choose the primer ?

07 May 2024 8,812 2 View

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