04 April 2016 2 9K Report

Hi Everyone,

I am new to NGS and performing microRNA analysis;

The organism I am working with does not have a reference genome as of now. Though in my case I do have some EST fasta sequences present for my organism (I have downloaded them from NCBI)

I have already done iterative mapping of my samples using the EST sequences as my reference genome. (I am not sure if this protocol is correct; Let me know if I am going in the wrong direction)

Which tool can I use for further analysis?

I want to find out known microRNA families, known miRNA variants, small RNA length, novel miRNAs, targets of novel miRNAs, structure of precursors of novel miRNA, GO ontology, KEGG pathway...

Any suggestions are welcome

 

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