Dear colleges,
I am trying to extract microbial DNA from a food product (feed) and I have not any equipment for mechanical lysis. I have checked enzymatic protocols and most of them use TE buffer and lysozyme, but I have found a wide diversity of TE buffer compositions and lysozyme concentrations.
Can anyone facilitate me instructions for TE buffer and lysozyme use for DNA extraction protocol? (both volume and concentration).
Thank you in
The following links are related to your query. I think so it may useful.
https://www.quora.com/How-is-TE-buffer-used-to-extract-DNA
file:///C:/Users/TEMP.DESKTOP-LVKSUC2.011/Downloads/TEbuffer.pdf
https://www.quora.com/What-is-the-role-of-a-TE-buffer-in-DNA-isolation
http://www.nhm.ac.uk/content/dam/nhmwww/our-science/dpts-facilities-staff/Coreresearchlabs/te-buffer.pdf
https://sciencing.com/function-tris-buffer-dna-extraction-6370973.html
Hi dear
this link can help you
https://www.researchgate.net/post/Microbial_DNA_extraction_from_feed_without_mechanical_lysis
Jannathulla and Haifa, thank you very much for your recommendation. I will check the links.
Miriam Cabello-Olmo, could you please suggest me which protocol you used to extract total microbiome DNA from feed, I will use fish feed for my work.
Thanks in advance.
- Badges
- Science method