We have planned to inject BrUrd into mice and, 3 hours later, to take tissue samples for histological examination and RT-PCR analysis. Might such a substance interfere in the detection of specific RNA sequences?
In training therapist and future social service providers I have come across the bias that the more attractive a client, whether it is physically or in accommodating or feeding into the receivers...
26 March 2019 6,674 0 View
Dear colleges, I am trying to extract microbial DNA from a food product (feed) and I have not any equipment for mechanical lysis. I have checked enzymatic protocols and most of them use TE buffer...
09 October 2017 4,001 4 View
We are trying to grow LABs but we have some problems to create the anaerobic conditions. In our study we only attempt to compare the microbial composition of a food product through time, and we...
22 August 2017 5,607 6 View
I wish to analyze the bacterial composition in a food sample and I wanna use a liquid enrichment medium prior to agar media. I have been looking for but I am not sure which broth medium would...
12 June 2017 2,917 4 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View