Visualizing cell pellet after FACS?

Hi all, I am sorting small number of cells (10,000-50,000)in 1.5 ml tubes using FACS. I am unable to see pellet of cells in the tube. I understand that it is difficult to observe pellet with such...

01 February 2018 1,261 7 View

18s Ct and RNA quality

Hi all, I FACS sorted 50000 cells and Isolated RNA from them using RNAeasy plus micro kit. Isolated RNA showed 260/280 ratio of 2.08 but, 260/230 ratio is 0.1. Conc. was 3ng/ul. we dont have...

07 August 2017 5,445 3 View

What can be Lineage-CD45+SCA-1+ positive Cells in brain?

Hi all, I am working on innate lymphoid cells and found that in our settings we have significant Lin-CD45+SCA-1+ cells. I further analyzed these cells for the expression of THY1, KLRG1, IL7ra and...

06 July 2016 2,191 5 View

How to resolve IL7Ra population in mouse spleen?

03 April 2016 528 5 View

Lineage cocktail for studying mouse ILCs?

01 February 2016 7,211 4 View

Microglial alpha7nAchR expression

Dear all, I am interested in knowing the expression of a7nAchR on micoglial cells in the brain, however I am unable to find good literature/figure showing expression of a7nAchR on micoglial cells...

01 January 1970 2,953 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View