7 Questions 12 Answers 0 Followers
Questions related from Arshed Nazmi
Hi all, I am sorting small number of cells (10,000-50,000)in 1.5 ml tubes using FACS. I am unable to see pellet of cells in the tube. I understand that it is difficult to observe pellet with such...
02 February 2018 1,321 7 View
Hi all, I FACS sorted 50000 cells and Isolated RNA from them using RNAeasy plus micro kit. Isolated RNA showed 260/280 ratio of 2.08 but, 260/230 ratio is 0.1. Conc. was 3ng/ul. we dont have...
08 August 2017 5,484 3 View
Hi all, I am working on innate lymphoid cells and found that in our settings we have significant Lin-CD45+SCA-1+ cells. I further analyzed these cells for the expression of THY1, KLRG1, IL7ra and...
07 July 2016 2,235 5 View
Dear all, Are there any mice strain available which are deficient specifically in group 3 or group 2 ILCs? best, Arshed
04 April 2016 4,196 4 View
Dear all, I am using IL7Ra antibody (cllone A7R34) PEcy7 conjugated to stain for ILCs in mouse spleen but, I am not getting well resolved population of IL7Ra positive cells. I want to improve...
04 April 2016 567 5 View
Dear all, I am using following Abs as Lineage dump channel. Is this cocktail ok to use for mouse ILCs? Anti-Mouse CD3 (17A2) FITCAnti-Mouse CD45R (B220) (RA3-6B2) FITCAnti-Mouse CD11b...
02 February 2016 7,251 4 View
Dear all, I am interested in knowing the expression of a7nAchR on micoglial cells in the brain, however I am unable to find good literature/figure showing expression of a7nAchR on micoglial cells...
01 January 1970 2,993 1 View
