Hi all,
I am working on innate lymphoid cells and found that in our settings we have significant Lin-CD45+SCA-1+ cells. I further analyzed these cells for the expression of THY1, KLRG1, IL7ra and Nkp46 and found that there is big population (60% ) of them are negative for the expression of Thy1.2, KLRG1 and Nkp46. They are not positive for IL7ra either. In short , my question is what these Lin-CD45+SCA1+ Thy1-KLRG1-IL7ra-NKP46- cell can be?
Hi Arshed
This recent article may be of interest to you.
http://www.ncbi.nlm.nih.gov/pubmed/26949018
Kim HS et al. A novel IL-10-producing innate lymphoid cells (ILC10) in a contact hypersensitivity mouse model. BMB Rep 2016 May;49(5):293-6.
Abstract
The immunoregulatory cytokine Interleukin 10 (IL-10) protein is produced by various cells during the course of inflammatory disorders. Mainly, it downregulates pro-inflammatory cytokines, antigen presentation, and helper T cell activation. In this study, we show that the ratio of IL-10-producing cells was significantly increased in lineage negative (i.e., not T, B, or leukocyte cell lineages) cells than in lineage positive cells in lymphoid and peripheral tissues. We further observed that IL-10-producing innate lymphoid cells (ILCs), here called firstly ILC10, were increased in number in oxazolone-induced contact hypersensitivity (CHS) mice. In detail, IL-10-producing lineage negative cells were elevated in the axillary, inguinal lymph node, and ear tissues of CHS mice. Notably, the cells expressed classical ILC marker proteins such as CD45, CD127, and Sca-1. Altogether, our findings suggest for the first time that ILC10s are present in various physiological settings and could be involved in numerous immune responses as regulatory cells. [BMB Reports 2016; 49(5): 293-296].
Regards, Rosemary
http://www.ncbi.nlm.nih.gov/pubmed/26949018
Hi Rosemary,
Thanks for this interesting article but, my cells of interest are not positive for CD127, hence cannot be considered as ILCs. Can they be hematopoietic stem cells?
Hi again Arshed
A few questions. Which tissue(s) did you find these cells? What proportion of the CD45+ cell population are these cells? Were the mice a particular strain or genetically modified? Had the mice been treated in any particular way?
Hi,
Its Brain tissue from wild type C57BL6 mice. its approx 2% of all cd45 cells (25% of live cells). No they are not treated.
Hi again,
That your cells are from brain tissue is an important piece of additional information. I have not worked on brain tissue. It is a very specialised area with many technical challenges. No doubt you have ruled out that your cells are some sort of precursor microglia (brain macrophages)? It is surprising that they are 2% of the CD45+ population.
You might consider adding 'brain' to your keyword list so that your question reaches the ResearchGate 'brain' forum too.
Good luck - I hope you find an answer.
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