Visualizing cell pellet after FACS?

Hi all, I am sorting small number of cells (10,000-50,000)in 1.5 ml tubes using FACS. I am unable to see pellet of cells in the tube. I understand that it is difficult to observe pellet with such...

01 February 2018 1,261 7 View

18s Ct and RNA quality

Hi all, I FACS sorted 50000 cells and Isolated RNA from them using RNAeasy plus micro kit. Isolated RNA showed 260/280 ratio of 2.08 but, 260/230 ratio is 0.1. Conc. was 3ng/ul. we dont have...

07 August 2017 5,445 3 View

What can be Lineage-CD45+SCA-1+ positive Cells in brain?

Hi all, I am working on innate lymphoid cells and found that in our settings we have significant Lin-CD45+SCA-1+ cells. I further analyzed these cells for the expression of THY1, KLRG1, IL7ra and...

06 July 2016 2,191 5 View

How to resolve IL7Ra population in mouse spleen?

03 April 2016 528 5 View

Mice deficient in ILC3?

Dear all, Are there any mice strain available which are deficient specifically in group 3 or group 2 ILCs? best, Arshed

03 April 2016 4,157 4 View

Microglial alpha7nAchR expression

Dear all, I am interested in knowing the expression of a7nAchR on micoglial cells in the brain, however I am unable to find good literature/figure showing expression of a7nAchR on micoglial cells...

01 January 1970 2,953 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View