Methylation analysis in Mycobacterium tuberculosis complex

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Liying Yan

Do you plan to sequence the entire bacteria genome or a particular gene in the genome? Pyrosequencing or targeted NextGen bisulfite sequencing is recommended.

Sara Al Mahruqi

Specific motifs. avoiding NGS platform to lower the cost

For a specfic motifs, Pyrosequencing may be a good choice? How many samples to be analyzed?

Around 100 samples. Can you share protocol if you have one?!

I need guidance in designing primers for these motifs, kindly suggest reading material

You can Google Pyrosequencing methylation analysis and get the information. Do you mind sharing the target seuqence? If you prefer, we can discuss the method offline. My email address is [email protected]. Do you have an access of a Pyrosequencer?