Can anyone give me Protocol for determination of cellulose, hemicellulose and Lignin in fruits? Do ADF and NDF methods can be used for fruit ?
Is there any free software for caring out these calculations.
21 October 2023 4,961 0 View
this will be done to knoe fibre composition
19 September 2023 8,199 2 View
Hi everyone, I’m using hemoglobin in the lab and I need it to be in the reduced state (so it is able to bind with CO2). The hemoglobin I have is a lyophilized powder from Sigma Aldrich (H7379)....
13 September 2023 9,133 4 View
Hello, I need to use guanidium chloride to denature a protein. However, I’m reluctant because it is absolutely crucial for my experiment that no isocyanic acid is formed. I need to know the...
13 September 2023 4,587 1 View
i have 100NIH units of thrombin and its molar mass is 37.4kDa, how to make the solution and how do determine it's molarity?
27 July 2023 6,359 0 View
Please suggest any other method for estimating Heterogenous Panel Vector AutoRegressive thus allowing individual irf to be computed.
12 June 2023 9,061 3 View
Dear Researcher, I am working on HIT_T 15 cells and I want to measure the intracellular calcium concentration using Fura_2am, I found many protocols using probenecid , My question: is it ok if i...
17 April 2023 6,450 0 View
Hi, Can anybody explain, which grade DMSO can be used for diluting plant extracts for MTT assay? The cell cryopresevative DMSO? and what to do if the extracts are not completely dissolved in...
04 April 2023 4,268 1 View
I am trying to build an amide bond between COOH functionalized aptamer and small molecule. On that purpose I need EDC/NHS solution for establishing amide bond, using MES and PBS buffers,...
28 March 2023 634 6 View
I made a mistake, I seeded my cells in 6 well plates instead of 96 well plates. my question is can I transfer my cells to 96 well by using trypsin is that possible?
12 February 2023 7,826 0 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
What are the best practices for washing and preparing fruits and vegetables to reduce pesticide residues?
27 July 2024 9,147 4 View
I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...
15 July 2024 9,827 1 View
Hello everyone reading this topic! It is well known that microcrystalline cellulose 102 is recommended for use in direct compression technology, however, like other grades, it has poor flowability...
08 July 2024 2,807 0 View
Type 2 diabetes can be managed and, in some cases, put into remission through proper diet and exercise, regardless of age. Here are some key points: Weight Management: Achieving and maintaining a...
07 July 2024 8,745 2 View
I use X-ray diffraction (XRD) techniques to quantify the degree of crystallinity in my samples, referring to the following formula (CrI=Acrystal-Aamph/Atotal ). using deconvolution area methods (...
06 July 2024 6,234 9 View
Does produce in season have more nutrients when eaten in season and fruits and vegetables safe to eat if they have been treated with pesticides?
02 July 2024 4,144 2 View
What is meant by cross-contamination and what causes fruits and vegetables to become contaminated during preparation and storage?
02 July 2024 9,204 0 View
What is value addition of fruit and vegetable through processing and role of value addition in agriculture?
02 July 2024 2,507 4 View
Does vinegar remove pesticides from fruits and vegetables and best ways to treat fruits and vegetables in order to get rid of the pesticides?
02 July 2024 4,651 1 View