Dear researcher,

You can use the UV-Vis spectroscopy method and/or TG analysis for finding encapsulation efficiency. You can study the below articles for more information:

Article Synthesis and evaluation of polyethylene glycol- and folic a...

Article Targeting delivery of Oxaliplatin with smart PEG-modified PA...

Fluorescence. Doxorubicin can be detected by a fluorometer.

I suggest you to release the liposome before quantification and to prepare a standard curve on Doxo with broken liposome to correct for the background noise.

Regards

hiii

you could measure the DOX with fluorimetry, as well.

good luck

Francesco Mainini Thank you for your reply. I have another question if you don't mind.

Based on some papers Liposomal DOX was separated by centrifuge 13000-15000 rpm for 15 mins 4C.

Will that speed be enough to separate the capsulated from the free DOX?

Also will it agglomerate or disrupt the liposomal pellet?

Thanks,

Roghayyeh Vakili-Ghartavol Thank you so much.

Asghar Narmani Thank you so much.

Dear Tia,

I believe dialysis would be the best methodotology to remove unloaded DOX (and with most compounds loaded in liposomes). The problem with centrifuge is that you will end up with agglomarates and a bad pdi most likely.

Francesco Mainini Thank you so much for your answer really appreciate it. I'll check an appropriate protocol and use dialysis.