I centrifuged liposomal ( ascorbic acid in PBS/ Alpha lipoic acid in Eth ). Discarded the supernatant and trying to resuspend the pellet (encapsulated drug) in PBS to be used for cancer cell assay. The pellet is so hard to dissolve or resuspend in PBS I used vigrous vortexing but still agglomerated or caked pellet doesn't resuspend. I used DSPC: CHOL (1:1) molar ratio (Eth: PBS) nanoAssemblr benchtop.
-Shall I decrease the centrifuge speed or time?
-Any suggestions for using other solvents that won't disrupt the liposomes or have an impact on cell testing ?
Thanks,
You should try to use one of the following:
1- Decrease the speed and/or time of the centrifugation.
2- Sonication for 5 min.
Also, you should know that the centrifugation method could alter several characteristics of the liposomal suspension like mean diameter and encapsulation efficiency. So, I prefer to use the dialysis method to separate the entrapped drug.
I think the use of surfactant is inappropriate in our current case.
Hi there,
For the separation of free drug, you can use the dialysis method based on dialysis bag.
Also, decrease the speed and/or time of the centrifugation, could be helpful.
my regards.
- Badges
- Science topic
- Similar topics
- Philosophy
- Philosophy Of Science
- Reasoning