Tia Mo

9 Questions 14 Answers 0 Followers

Questions related from Tia Mo

No statistical significance but 70% cell inhibition between the treated group and the control (MTT assay) ?

I'm treating cancer cells with two different cytotoxic combination drug with low concentrations (mtt assay). I have 5 concentration (serial dilution 1:2) of each drug in triplicates. Drug A, Drug...

30 March 2022 4,522 2 View

How to resuspend caked or agglomerated liposomal pellet after centrifugation?

I centrifuged liposomal ( ascorbic acid in PBS/ Alpha lipoic acid in Eth ). Discarded the supernatant and trying to resuspend the pellet (encapsulated drug) in PBS to be used for cancer cell...

02 February 2022 2,435 4 View

Method for measuring the encapsulation efficiency of liposomal Doxorubicin rather than using HLPC?

Would you please suggest a simple and rapid protocol/method for measuring the encapsulation efficiency of liposomal Doxorubicin(Cholesterol: DSPC) for example UV/centrifugation techniques. P.S. I...

28 December 2021 8,263 8 View

can you help me determine the encapsulation efficiency of liposomal ascorbic acid using a fluorescent dye?

I need a dye that wouldn't interact with the ascorbic acid to be encapsulated as a marker for measuring the encapsulation efficiency using the UV spectrometer. I have tired to use some dyes but it...

15 June 2021 2,624 5 View

Suggestions to drug similar to doxorubicin (as a model drug) for liposomal preparation co- loaded with antioxidant?

I'm currently preparing a liposome loaded with Ascorbic acid and doxorubicin both in aqueous media. I have prepared liposomal ascorbic acid using microfluidics and was planning to add the...

15 June 2021 2,210 3 View

High zeta potential value for liposomes (Malvern zetasizer)?

I did a liposomal preparation of DPPC:CHOL 1:1 with flow ratio 2:1 (PBS: Ethanol) based on literature of review using microfluidics nano-assembler bench top. I measured the size using Malvern...

07 May 2021 1,069 8 View

Reason for extremely high zeta potential for liposomes?

I did a liposomal preparation of DPPC:CHOL 1:1 with flow ratio 2:1 (PBS: Ethanol) based on literature of review using microfluidics nano-assembler bench top. I meaured the size using Malvern...

19 November 2020 3,467 14 View

Significant change in liposomal size when switched from distilled water to PBS buffer 7.4?

I have prepared DPPC:Chol liposomes 10mg/ml 1:1 weight ratio with microfluidics nanoassemblr bench top with FR 1:1 and TFR 12ml/min. Solvents used (Ethanol: Distilled water).The size on Malvern...

12 November 2020 224 5 View

How do I choose the right purification method for my liposomal preparation DPPC:CHOL?

I'm new in the field and I prepared a liposomal preparation using microfluidics (Nano assembler) DPPC:CHOL (2:1), solvents ethanol: distilled water. No drug loaded since I'm training. How do I...

27 October 2020 3,421 5 View