Please assist on this
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,705 1 View
I am trying to understand the different phosphorylation states of SRSF1 protein in the cytoplasm vs nucleus and looking for specific antibodies for the phosphor state. As SR proteins include...
11 February 2024 7,242 3 View
the company that I'm working in produces sauce and we notice there is watery issue in our oyster sauce after bottling although the sauce is within its validated shelf life.
09 May 2023 5,977 1 View
I transfected HCt116 cells with GFP using Lipo3000. I didn't change media after transfection as the manufacturer guidelines say not necessary. However, I noticed a change in the cell morphology...
28 April 2023 6,071 6 View
I have done potentiodynamic polarization (Tafel) studies for a few surface modified samples. The intercepts and slopes were done exactly by the origin software. Its results show a gradual...
06 April 2023 1,226 1 View
I am trying to study the RNA-protein interactome of a specific protein using IP studies. I need to use native IP for my purpose and wanted to know if native RIP is without cross-linking study and...
01 February 2023 915 0 View
I am comparing the protein expression levels in subcellular fractionations (Cytoplasm and nuclear). If I load an equal volume of both extracts (15 ul each to individual wells), is this still valid...
26 January 2023 6,257 3 View
I want to know tutorial help to design and how to choose suitable g for knok out
29 July 2022 4,099 2 View
I want to design g RNA for knock out pathway gene to enhance secondary metabolites
29 July 2022 8,077 0 View
Is DMEM or DMEM/F-12K the right media for culturing HCT116 cells apart from McCoys media? Did anyone use the gibco one? I see a lot of dead cells when using DMEM. It would be helpful if someone...
20 June 2022 1,059 3 View
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
30 July 2024 3,294 3 View
Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?
25 July 2024 5,482 2 View
Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...
16 July 2024 7,937 1 View
we have a nafion 117 membrane and we want to know if it has the properties claimed by the sellers. what are the tests available ?
13 July 2024 503 1 View
NA
11 July 2024 8,969 1 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
I have this problem with my western blot for the ABCG2 antibody on nitrocellulose membrane. Help please
02 July 2024 8,715 2 View
I am trying to insert cholesterol inside the DPPC membrane by using gmx insert_molecules -replace. But number of DPPC molecules replaced is much higher than the number of cholesterol inserted?...
30 June 2024 4,160 2 View
Is there an established protocol to measure the permeability of bacterial membranes? Thank you
30 June 2024 6,614 3 View
Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...
29 June 2024 9,688 1 View
