Hi everyone,
I want to measure prebiotic activity of a fruit powder ( using fecal microbiota), I am wondering if I measure short chain fatty acids at the end of the test , how can I know that SCFA is produced as result of using prebiotc (fibers) by probiotics and NOT by other microbiota, and also NOT from consumption of other components like glucose fructose of the fruit? Many thanks for your help as I am very armature in probiotc topics.
Also advises on choosing the approprite probiotic would be appreciated.
Hi,
initial you must fat extract from your production such as yogurt second step, short chain fatty acids is determining by use GC-MS technique.
Hi..you might use the negative control (blank), glucose and fructose samples too to be compared, then you can have the idea of their fermentation. i think it is quite difficult to measure the SCFA production from one single colony (except for pure cultures) because the SCFA produced in the fecal microbiota is a complex interaction between several species. Because, bacteroidetes also ferment simple sugars derived from breakdown of complex carbohydrates to organic acids including SCFA and hydrogen. Secondary fermenters such as Clostridium species and butyrate-producing bacteria further utilize the organic acids to generate additional SCFA.
Dear Napisah, Many thanks for your help, I have decided go with single strain of probiotic, but first of all I dont know if its ok if I choose any probiotic strain like Lactobacilus for testing prebiotic activity of my sample or depending on the sample the type of probiotic we choose varies? Also I am not sure how to set the positive control and calculate it.
Hi Pery Perv, If you want to proceed with one single strain, i think you should choose one potential probiotic which means either form known lactobacillus or bifidobacterium strain. Like my study, i have tested 23 strains of LAB (from many sources like ATCC, FTCC and FTDC) and calculate its Prebiotic acitvity score. Then from there i can see the pattern.
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