What is the difference between master and doctoral degree?

Hello there.. I've recently submitted my master thesis in marketing. As a reseacher, I now know that the purpose of a master degree is to know academic research tools and how to do and write an...

09 February 2021 6,692 9 View

How can I reduce run-to-run variation in TMT MS experiments?

I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...

31 January 2021 3,405 3 View

Can I use alpha amylase enzyme from (fungal source) if I need to characterize alpha amylase inhibitor from marine algae for hyperglycemia management?

I need to characterize alpha amylase enzyme from macroalgae for management of blood glucose level in diabetic patients, my question is can I use (fungal alpha amylase enzyme) in inhibition assay?

18 January 2021 735 4 View

How can I write a Scientific proposal to present it in public conference?

Writing Proposal

30 December 2020 6,783 3 View

Why RNA is totally degraded?

18 October 2020 5,252 22 View

How can i separate a polymer after a polymerization reaction from the unreacted monomers, taking in consideration that it's really sticky?

The polymer i'm fabricating will be used to make a delivery system, so it must be separated from the monomers right ? i tried to use solvent antisolvent method but the polymer is only soluble in...

13 October 2020 2,002 4 View

How to prepare 37% Formaldehyde from paraformaldehyde?

I want to prepare 37% formaldehyde for denatured formaldehyde agarose gel preparation (for RNA) How can I prepare 37% formaldehyde? And how long this solution would be stable?

11 October 2020 914 4 View

What is the concentration of saturated solution of bromophenol blue?

I want to know the concentration of a home-made saturated aqueous solution of Bromophenol blue. How could I know when I add unknown number of grams of the dye?

05 October 2020 3,423 3 View

What is the reason of introduction of SDM 6 nucleotides before the right position in a gene on a plasmid when sequenced?

I am trying to introduce SDM for a gene on a plasmid. After Pcr (with HF Q5 polymerase and primers designed by NEB website the length of one of them 19) , Dpn 1, ligation, transformation and...

27 September 2020 5,493 11 View

What practical resources could be used to learn animal experimentation?

Hi There, I need any practical resources like a textbook, handbook, series of videos to learn the basics techniques of running an animal study to test drug efficacy. points have to be covered...

14 January 2020 6,564 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View