What are the important tips for writing review articles?

Important tips for writing a good review articles

24 February 2021 8,544 6 View

Does Lucifer Yellow interfere with miniEPSC/IPSC recordings?

I want to mark the neurons I am recording from during whole cell Patch clamp. I am thinking of using Lucifer Yellow. Does it increase noise during recording? What are the better alternatives to...

04 January 2021 7,791 3 View

How Learning in Multiagent Control System Using Graph Theory is useful ?

I would like to know application of learning and control in MAS setting. And where could I find simulation codes of learning in MAS control

04 January 2021 1,866 4 View

Kitagawa Diagram is stress controlled or strain controlled?

This is required since the stress v/s crack length has to be determined

23 December 2020 3,446 1 View

Is the expression of House keeping genes disturbed in an abnormal situation like in a diseased tissue?

In gene expression studies, what would be the reliability of house keeping genes expression say in a disease/insect attacked tissue that is being subjected to studies (RT qPCR ). Will the...

14 December 2020 3,445 5 View

Could anyone suggest me what is the best way/method to isolate total RNA from the platelets?

I have tried to isolate platelet total RNA from the human and sheep blood using TRIzol reagent. After isolation of platelets from the blood samples, total RNA extracted, and quantified using...

02 December 2020 6,165 4 View

How to analysis drought in lab condition in wheat?

I wish to conduct the drought analysis study in wheat. anyone knows Procedure to conduct drought analysis, especially in wheat crop. Please share the information. I am looking to develop training...

09 November 2020 3,850 4 View

What will happen if people are able to make their own smartphones?

09 November 2020 8,056 7 View

How can we stop mental depression?

27 October 2020 4,429 6 View

What is the difference between PID-controller design based on retrend / detrend model ?

Dear all, I have a set of I/O data. I estimated a transfer function based ( command: tfest) on this data in MATLAB. (I removed the trend from the data before it: command: detrend). Can I...

24 October 2020 1,664 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View