Mammosphere staining protocol?

14 January 2021 1 10K Report

Hello,

does anyone have or can suggest a reliable protocol for immunofluorescent staining of mamospheres grown in suspension at low-attachement plates? Thanks in advance!

Best,

Theo

Salah Uddin

See the attached paper related to your topic

Dr. Salahuddin

Badges
Science method

More Theodoros Karalis's questions See All

What are these structures? Are they organisms?

Scale bar is 100 μm and their lengths vary from 130 μm to 240 and 350 μm. Image is taken by optical microscope. Samples might have been in contact with dirty (pond, river) water.

27 October 2023 6,052 2 View

Do AspN and GluC cut at their D and E residues if there's a proline P just before that?

Do AspN and GluC cut at their D and E residues if there's a proline before their cutting residue? I know trypsin can't cut if there's a proline so I'm wondering if that's the case for these...

16 August 2023 7,840 0 View

What's the size of Calu-3 cells (diameter, μm)?

I'm planning to run a single cell analysis experiment using the C1 Fluidigm Instrument. In my case, there is a limitation on the size of the cells that can be captured by the instrument 10-17um. I...

21 July 2023 2,193 3 View

What temperature should my feed have in a methanol-water distilation column?

Hallo and thank you in advance. I am not an engineer, I am a chemist, and my knowledge of distillation processes is limited. In the plant I work we face a lot of problems with our methanol...

26 February 2023 3,011 0 View

Is naive CD4 T cell differentiation to 2 different subtypes in the same well possible?

Has anyone tried culturing naive CD4 T cells on a medium containing cytokines for both Th17 & Treg differentiation? What was the outcome?

14 November 2022 2,755 0 View

What is the correct way to apply a cutoff for the Coulomb potential when simulating bulk water?

I am trying to simulate bulk water using various models based on the Lenard Jones potential. This also includes a Coulomb potential between atoms belonging to different molecules. When using a...

05 January 2019 6,823 2 View

How to evaluate if a transcriptional co-activator binds to transcription factors regulating the expression of a certain gene?

Greetings to all, i want to study the role of a transcriptional co-activator in the regulation of a certain gene expression, through binding to specific transcription factors. In other words, i...

03 November 2017 1,012 4 View

How to deactivate silica?

I would like to use silica sand as a substrate to immobilize a compound and then disperse it in water for extraction purposes. However, silica sand adsorbs my target compounds (unwanted...

04 October 2017 4,804 5 View

Large Cancer Cells in Culture

Hello everyone, often i observe large cells (approximately 4 or 5 times the size of the other cancer cells) in my cell culture. Does anyone has a clue what's happening with these cells? Also i...

21 June 2017 9,982 2 View

What should be the ideal waveform of a compound action potential recorded from the surface of the Vagus Nerve?

Are there any biophysical models simulating activity traveling through some fibers (or fascicles) and how this would project on the surface? If there are no details models, what would be an...

05 October 2016 1,289 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View