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Questions related from Theodoros Karalis
Hello, does anyone have or can suggest a reliable protocol for immunofluorescent staining of mamospheres grown in suspension at low-attachement plates? Thanks in advance! Best, Theo
14 January 2021 10,121 1 View
Greetings to all, i want to study the role of a transcriptional co-activator in the regulation of a certain gene expression, through binding to specific transcription factors. In other words, i...
04 November 2017 1,037 4 View
Hello everyone, often i observe large cells (approximately 4 or 5 times the size of the other cancer cells) in my cell culture. Does anyone has a clue what's happening with these cells? Also i...
22 June 2017 10,018 2 View
Hello everyone,recently i am performing immunofluorescence analysis experiments and i came across the anti-HAS2 goat polyclonal antibody (S-15, sc-34067) from santa cruz biotechnology, but i am...
21 June 2016 3,300 1 View
Cheers,can anyone specify an optimum dilution for Streptavidin-Alexa Fluor 488 "green" to be used in immunofluorescence? [I am using Streptavidin-Alexa Fluor 488 , Molecular Probes, S11223]
21 May 2016 9,545 6 View