Hi all,
I was originally using a RotorGene 6000 machine that was last serviced 6 years ago. It kept giving me strange results so I sent the run files to QIAGEN for their tech reps to have a look. They wrote back saying the machine has issues with its heating/cooling system.
Now I am using a different real time PCR machine and the results are much more coherent. As I near the end of my research project, I want to devote a chapter on my thesis on proper care of real time PCR machines.
My question is; is there an established guideline for the proper maintenance of qPCR machines? When do we start discarding results from a poorly maintained machine, has anyone published a paper on it?
I have searched PubMed with the key words "maintenance of real time PCR machine" but yielded no hits.
Thanks!
Yes, I found the PDF file but I was wondering if there was any study conducted into the accuracy of real time PCR machines if it hasnt been calibrated for a long time. I have heard that in forensic medicine, their real time PCR machine has to be calibrated every six months and daily validation experiments must be conducted daily, if not the evidence is inadmissible in a court of law.
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