Macrophage differentiation/polarization on uncoated glass coverslips

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Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

Beta galactosidase assay in milk?

I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...

12 June 2024 7,331 1 View

Does the first semicircle represent contact resistance, and what is the reason for its generation?

My research topic is the corrosion of gray cast iron in methanol. EIS measurements were conducted at open circuit potentials (Eoc) using a sinusoidal signal with the amplitude of 10 mV from 100...

05 June 2024 6,891 2 View

What to do if Cronbach’s alpha is unacceptable on the Big Five Inventory (BFI-10)?

Internal consistency for Neuroticism, Extraversion, and Openness to Experience, Agreeableness, and Conscientiousness subscales is unacceptable (Cronbach's alpha = 0.49, 0.50, 0.45, 0.05, 0.40)....

04 June 2024 6,224 0 View

Co-incubation of neutrophils with exosomes?

Hello! I am planning on doing co-incubation experiments of neutrophils with exosomes and wanted to ask what concentration of exosomes, cell number and incubation time works with this cell...

29 May 2024 6,551 0 View

How to simulate plasma jet by COMSOL?

In COMSOL, how to use the gas concentration distribution results obtained by steady-state calculation as initial condition for plasma module for subsequent calculations? The commonly used data...

09 May 2024 7,335 0 View

How to analysis Mossbauer Spectroscopy data?

Scenario: I got two data files (α-Fe and Fe-containing samples), and the file names contain the max velocity value. Confusion: 1. How should I plot the X-axis? Is +/- velocity with points...

07 May 2024 3,244 0 View

How to extract bioactive compounds from fungal cultural filtrates...? Which of the techniques are best for the isolations...?

simply asking about the isolation of the pure compounds of fungal cultural filtrate as like the distillation of the essential oils..

06 May 2024 2,110 3 View

PCR & electrophoresis (confirmation) failing?

My fellow Academic colleagues! I together with my lab mates have a PCR-related issues that we hope that some(one) of you might have encountered and hopefully solved. In “short”, our initial PCR...

04 May 2024 8,788 5 View

What are the key differences between MMGBSA and MMPBSA in estimating free binding energy of a protein-ligand complex following Drug design study?

Please help to understand the aims and scopes of these two different techniques along with the possible computational cost that they render. Thanks.

25 April 2024 9,711 2 View

Fabrication of narrow glass capillary (inner diameter 200~500 nm)?

I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?

08 August 2024 8,915 0 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

Human macrophages cell surface markers?

I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...

29 July 2024 236 2 View

What is the right voltage sweep direction for characterization of HER kinetics?

In general, when performing a Tafel analysis for corrosion resistance measurements, a line voltage scan is performed from say -250 to +250 mV vs. OCV at low scan speed around 0.125 mV/s. However,...

25 July 2024 7,982 3 View

Detaching human-derived monocytes?

Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...

20 July 2024 7,922 2 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Differentiation THP1 cells into M2 macrophage with IL4 and IL13?

Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...

15 July 2024 5,153 2 View

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View