Macrogen´s Sequencing service

More Leandro A Sánchez's questions See All

Is there a good cleaning protocol available for C18-SPE material?

Hello !Does anyone know a good cleaning protocol to re-use C18 SPE material ?. We are currently using a glass column and want to recycle C18 solid phase, but we are not sure which procedure we...

09 October 2014 6,775 11 View

Where can I get Pseudomonas meridiana type strain MTCC 4993?

I'm looking for Pseudomonas meridiana type strain MTCC 4993. I could not get any response from the indian collection (MTCC). Maybe somebody here is working with this strain and knows how to get it.

07 August 2013 7,452 1 View

Does anyone know what might be the presence of halos around bacterial colonies when they grow in R2A saline medium?

When I grow the colonies in R2A medium, I saw clear halos surrounding the colonies. The medium is prepared with seawater and maybe some salts are precipitated after sterilization. Could this be...

04 May 2013 1,246 1 View

Troubles with GyrB amplification

Hi everyone. I´m having problems to amplify the GyrB gene in bacteria. I did the DNA extraction, and I could amplify the 16s without problems, but when I try with GyrB I can´t get any product. I´m...

06 July 2011 4,248 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Leandro A Sánchez

I just received the answer from Macrogen, I´ll put it here, maybe somebody needs it too..

Greetings..!

Thank you for your inquiry. The prices are as follows :

1) Purification = 2 Euro per tube / 100 Euro 96-well plate

2) Gel-extraction = 4 Euro per band

3) Reaction = 4,50 Euro

Macrogen Europe

Syed Sameer A

$2 per sample is what they charge for purification

Nur Hidayah Hairul Bahara

Is macrogen any good in terms of the yeild obtained?