can anyone suggest me possible composition of a lysis buffer for Black soldier fly (Hermetia illucens). Preferably containing Urea.
Basically I will do protein quantification using Bradford assay after lysing the cells.
Kindly suggest.
Dear Debasree Purkayastha
Pls. find the attached files and the references, good luck
Hoping this will be helpful to your project
Regards
Prof. Houda Kawas
https://www.science.gov/topicpages/p/por+hermetia+illucens.html
https://peerj.com/articles/2563.pdf
https://worldwidescience.org/topicpages/i/intestinal+por+hermetia.html
http://pubs.acs.org/doi/abs/10.1021/ac60147a030?src=recsys
https://www.bioz.com/result/trizol%20reagent/product/Invitrogen
http://www.bioone.org/doi/abs/10.1603/0046-225X%282006%2935%5B1710%3ACOGBIL%5D2.0.CO%3B2?mobileUi=0&prevSearch=&journalCode=enve
http://www.nature.com/articles/srep29505?WT.feed_name=subjects_entomology
http://forskning.ku.dk/find-en-forsker/?pure=files%2F16916302%2FBioMicroWorld2009-Book-of-Abstracts.pdf
http://etheses.whiterose.ac.uk/4092/1/new%20Copy%20%20of%20SALAH%20FINAL2013.pdf
Article Nutritional composition of black soldier fly ( Hermetia illu...
Also
http://www.tandfonline.com/doi/full/10.1080/1828051X.2016.1260499?af=R
https://pubag.nal.usda.gov/?page=144&per_page=100&search_field=subject
http://basic.ub.ac.id/files/proceeding/proceeding-basic-2014.pdf
Dear Prof. Houda Kawas,
This was really a great help. Thanks for your valuable time and input.
Regards,
Debasree
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