Greetings to everybody,
Has anybody experience with luxol fast blue as a stain for Mallory's alcoholic hyaline? Could you show me some examples of positive staining?
Many thanks
Is one of the aflatoxin (afl) genes mutated/not expressed? If so, which one?
09 February 2024 2,465 4 View
The account I want to delete has an out of date email address for me.
16 November 2023 8,187 0 View
I am currently doing some R&D prototyping. I need following ingredients from Nissan Chemical: Nissan Sunever polyimide SE 7511L (ca. 100g/100ml) Sunever solvent from JSR...
13 November 2023 2,435 0 View
we are researching lion's mane
01 November 2022 7,115 0 View
Hi, I have one technical question. What is the best protocol for plasma separation if I haven´t got a refrigerated centrifuge? I found the protocol: Centrifuge the samples at 2000 X g for 15...
25 May 2022 5,366 0 View
Hi, Im using Rigaku Ultima 4 diffractometer. Cobalt source, Cross beam optics, paralel beam geometry, soller 5deg, variable div slit, stripe detex ultra detector. For the last several years...
22 April 2022 8,642 0 View
Hello, I'm planning to perform an in vitro translation experiment to evaluate a coding potential of an RNA transcript (cca 1500 nt), predicted to code for a 8 kDa protein consisting of 68 aa...
03 February 2022 9,400 1 View
Hello. We have Autolab PGSTAT302N and also Autolab PGSTAT101 in our lab. Both are connected to software Nova 1.11. Is it possible to use multiple pulse amperometric procedure in this type of...
01 December 2021 2,741 0 View
Could you recommend any software, open source is most welcome, for 1D integrated analysis of the subsurface thermal field, including heat flux analysis, based on borehole data, thermal...
20 October 2021 4,678 4 View
I cultivate keratinocytes isolated from the foreskin. Initially, after the isolation of keratinocytes, only single fibroblasts are visible, but after two passages, the fibroblasts cover most of...
13 July 2021 5,532 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View