Low yield IVT RNA ?

29 April 2022 1 7K Report

Im using mMESSAGE mMACHINE T7 ULTRA Transcription Kit to transcribe my DNA. It produced 30 ng/ul which is 750 ng although the kit promises to yield atleast 15-20 ug of RNA. Even the control DNA provided in the kit produced 6.2 ug RNA. I followed the protocol as it was instructed. Kindly share tips to increase the yield upto 10 ug atleast. TIA!

Lukas Anneser

That kit normally delivers. Have you tried extending the IVT reaction? I normally do 6 hours +.

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