I ordered a gene from europhins in double stranded form. According to them it's lyophilized dry powder but on the tube it is mentioned 27.5 ng/ul,.i wana know if it's dried then what is the mentioned concentration for and secondly how much volume of nuclease free water and TE buffer should i use to resuspend it?
I got plasmids from Genscript but not from Eurofins. Normally the tube with lyophilized material comes with instructions of how to resuspend the DNA. In your case if there is not instructions on how to resuspend the DNA, I would just. use 20ul of TE buffer and measure in the nano drop. Even if the dilution is too high (which I doubt with 20ul) you will be able to transform it into E.coli DH5a to prepare a glycerol stock and also minipreps to amplify your synthetic plasmid.
Normally europhin gives the quantity present in mol or nmol, check your tube. If you have the quantity you can know which volume to add. If not, I agree with Xi Jiang answer.
Hi there,
If the synthetic gene is cloned in a bacterial vector, the actual amount is not crucial. Resuspend the sample in a few µL of TE and use 1µL (or even less) to transform a cloning strain in order to amplify the plasmid. From the miniprep you will easily know the amount of plasmid by spectrophotometry.
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