Dear all,

I am trying to do an RNA extraction of different bacterial species with RNAeasy mini kit, protokol #4 (lysozyme lysis + proteinase K digestion). Unfortunately, I have a fairly low yield (50-100 ng/mcl) and a high content of gDNA (~20%).

Any ideas how to increase of RNA's yeld? Is it necessary to increase the concentration of lysozyme in the TE buffer ( current 15 mg/ml) or reduce the amount of starting material?

Thanks in advance