Does anyone routinely use BODIPY 581/591 C11 to look at lipid peroxidation by flow cytometry?
The theory is that unoxidised BODIPY fluoresces with an Em around 581nm but the oxidised form fluoresces green with an Em of around 510nm.
There are a couple of reports out there indicating oxidation can be picked up as an increase in signal in the 530/30 channel off the 488 laser, but this only excites at approx. 6% of the excitation maximum and I'm not sure how much of a signal will be picked up?
Wouldn't it be best to use a colinear system with both 488 (blue) / 561 (yellow-green) and look at the ratio between the two signals?
I'd be interested to know what systems are being used for this and details of the analysis please.