Dear community,
Happy new year! I have a very rudimental question to bother you with. I'm interested in the principles of epigenetics. All the cells I'm familiar with are in cell cycle, such as HEK293 cells and a variety of tumor cell lines. I became very interested in post-mitotic cells that are not cycling anymore but I couldn't figure out a good (and easy) model to work on. I thought about using BMDM, but then I realized that they need around 7 days to fully mature, after which they will stay healthy only for a couple of days. Essentially, I'm looking for a primary post-mitotic cell that is:
1. easy to culture for a long time
2. easy to acquire from companies or mice.
3. a good transfection host.
At this stage I don't have a lot of plans regarding epigenome editing yet, and above are the only criteria I have. May I ask for some recommendations?
Thank you so much and best regards,
Changxu Fan
Hi,
I wish you a very Happy New Year!!!
Going through your query and requirement, I'm not sure how much it will work for you. My understanding is pointing toward CARDIOMYOCYTES which are post-mitotic cells.
Now if can you can get or isolated mesenchymal stem cells and using 5-Aza and TSA, differentiate them to cardiomyocytes. Then those CMs are post-mitotic, primary cells. Well, 5-Aza and TSA itself are epigenetic modifiers that will keep the epigenetic regulation framework. You can grow and maintain till two week or sometime more.
Now all this will ONLY fit based on your broader area of research. Pardon me if I'm not able to address your exact query.
Regards
Saurabh
Reminds me of cells that are in permenant stasis after prolonged cell cycle arrest.
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