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Is it possible that under daily change of media, a contaminated cell culture may manifest no visual signs of contamination? If so, when the cells look healthy and normal, is it safe to use these...
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I've read that the Bunsen burner is not recommanded for the cell culture hood, however, it's been a tradition in my lab. Can anyone explain the pros and cons of using it? Thanks in advance.
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After nocodazole treatment for cell cycle synchronization, most of my cells became round, but didn't float. What happened to them? Can I continue to lyse them for coIP and western blot?
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