Hi Dear Researchers,
As you know in laboratory-scale LFIA tests, the stability of Abs within the test and control regions is low, to say up to two weeks. However, in commercial hCG LFIA tests, you can see long-term stability of up to two years. Does anyone have any suggestion for enhancing the stability of Abs within NC membrane?
It is difficult to match the performance of commercial products as manufacturers have very tight control over the conditions during production e.g. controlled humidity environments, with different conditions at different stages in the process. Most research labs are not able to replicate these conditions. However, if you have not yet tried it, you should certainly keep the product dry once produced, by adding silica gel to the LF strips. Are you adding sugar to the capture Ab? This can also help to stabilise the final product.
Dear nick
From my experience, drying the ab's on the membrane in 37C for 1-2hours then storing them in dry conditions (sealed pouch with desicant), will do the work,
Dear Nick Gee,
Thank you for your helpful comment. I am familiar with commerical process to some extent but not all details. I'll try it.
Dear Oded Babai,
It will help to preserve the wicking effect of NC membrane and adsorption pad but I think it's not the whole solution.
In my previes work, wo manufactured LF assays, after dispencing the capture & Ctrl (in PBS), we dried the membrane and kept them dry, the Conj pad & sample pad were with the blocking reagents. It was stable for at least a year. The problem might be related to the ab's, you should test several ab's & test them with eccelarated stability
Falk Fish What concentrations of sugar have you used?
I do use some sucrose in the buffer I keep my nanoparticle-antibody conjugate in.
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