Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Do you have papers on hindlimb myology of birds to share?

Hi all! we are looking for papers (or information) on muscles of the hindlimbs of birds (extant and extinct) that include the intrinsic muscles of digits. Any help will be more than welcome! Thanks!

22 February 2021 7,186 3 View

Marker Set for 3D Motion Tracking of Upper Arm during Pointing Task?

Does anyone have recommendations of a marker set for 3D motion tracking of the upper arm during a pointing task? Most studies I have come across have focused on reaching. I plan to use Vicon Nexus...

12 January 2021 3,198 1 View

Are there any way to make the comparison between different analysis of reliability possible?

10 December 2020 7,922 4 View

How do I calculate variance explained by each predictor in a multiple regression via SPSS?

I have 4 independent variables within a multiple regression model. I am trying to figure out the percentage of variance each individual independent variable contributes to the model using SPSS....

28 November 2020 583 3 View

Does anyone has suggestions to extract carbohydrates from E. coli?

Dear colleagues, We are interested in extracting carbohydrates fro E. coli and the protocol we've seen so far include too many hours in a sonicator. Does anyone has suggestions or experinece in...

26 November 2020 5,907 3 View

Is the statistical analysis performed correct?

Dear colleagues, I have some doubts about the correctness of the following analysis. A class of 79 students underwent three different teaching methods that chronologically presented themselves...

02 November 2020 4,030 4 View

Is there a validated english questionnaire to assess Achievement Motivation in Sport (Hope for Success and Fear of Failure)?

I'm looking for an instrument in english that does not measure goal orientations.

04 October 2020 3,255 5 View

How to calculate a-priori sample size for a repeated measures 2x2 ANCOVA?

I am planning an experiment 2x2 within-subjects factors and additionally I would like to consider a covariate, so that a 2x2 repeated measures ANCOVA is the intended analysis. Does anyone know how...

16 August 2020 985 4 View

How should I present microRNA expression data from a single sample (leaves)?

Hello! I have a small RNA-seq analysis from leaves, and I will validate the known and the novel miRNAs by qPCR. My question is how I should present the qPCR results from a single sample (leaves)...

30 July 2020 9,696 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View