Lentivirus cloning design - CMV and CAG promoter conflict?

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Cross Attention in Transformers: Standard applications of the same ?

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Is there a problem with my RNA pellet?

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Strugglling with m6A dot blot any suugesstion ?

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RNA Extraction Using Hot Borate Method No Longer Working?

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

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The Optimal Experimental Approach for Gene Function Analysis?

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Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

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Santiago Haase

With the enhancer two times in the vector, I would be concerned about intra-recombination in the in the production steps. The PGK promoter is commonly used in viral constructs, you may consider exchanging your CMV promoter for that one. WPRE also is used for GOI expression with good levels of transcription.

Simon N Waddington

I guess one option could be to use chicken beta actin since CAG is chicken beta actin plus the CMV early enhancer, but it nowhere near as strong without the enhancer. I've heard the GAPDH promoter is quite good, though I'd agree with Santiago that PGK is a pretty good bet