Length of a specific probe for DNA FISH?

More Mazen Katkout's questions See All

Is it possible to reset the chewing pattern cycle of a patient with chewing side preference in order to preserve condyle from damage?

09 August 2023 9,371 0 View

Stabilization Splint and TMD?

Q-1: If a placebo splint resolves symptoms of TMD, as proved by some studies, and therapeutic effect is also achieved with splints that are not in centric relation, what is the clinical...

01 January 2023 9,744 3 View

Does anybody know any physical argument for using zeta function regularization?

Does anybody know any physical argument for using zeta function regularization? Or it is an open question? Thanks.

26 July 2022 9,440 17 View

What is the source of quantized energy for the particle in box?

I want to talk about the stationary state and especially for the example of a particle in the box (infinite square well), even this is a simple example but in fact, it contains a strange behavior,...

13 June 2021 2,181 8 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,863 9 View

How to extract NLR genes from a database?

Greetings to all, I am not a bioinformatics person, but looking for a method that help me extract all NLR genes from benthamiana, tomato and soybean databases.. any suggestions so I can use my...

08 February 2021 4,884 2 View

About protocols used for glucose uptake in HepG2 cells?

Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...

25 January 2021 7,937 3 View

Measuring small RNA abundance, without sequencing?

Dear all, Do you know any reliable and fast method to measure the levels of plant small RNAs (21,22 and 24 mer)? I am interested in only comparing the abundance of those small RNA between mock...

21 January 2021 4,268 6 View

Why is my x-alpha-gal soltuion forming a white precipitate when added to water?

I prepared x-alpha-GAL solution in DMF at concentration 20mg/ml. When adding this solution to water (liquid media), a white precipitate is forming. I tried mixing DMF with water, NO precipitate. I...

12 August 2019 1,341 1 View

What is the capacity (insert size) of binary expression vectors?

Hello all, Is there any limit for the insert size in the binnary vectors such as pCambia 1300, pPZP, or pBin61 (used for agroinfiltration)? Big genes (around 4 Kb) are inserted correctly, but not...

03 April 2019 9,893 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Wolfgang Hennig

Principally this should be no problem and may be better than long fragments. However, as in all in situ hybridization experiments, it dependes on details of the material. What is the fixation? Whole mount or slide? What cell type? The details given in my in situ hybridization review in Intern.Rev.Cytol 36, 1973, are still valid

Sonal Rajiv Bakshi

It will depend on chromosomal location also, subtelomeric regions may not give good hybridization regardless of size.