Which stages in the QuEChERS extraction and clean-up technique are the most stable for storage over several days or weeks (e.g. when extracting polycyclic aromatic hydrocarbons (PAHs) from fish)? Thank you.
Many literatures have shown that the RVE model shows the grain boundaries between different grains. How can this be achieved using DAMASK+paraview?
21 July 2024 9,224 1 View
Been doing searches to find the best paper to start understanding the advantages and disadvantages of each technology: Gaussia Luciferase for Bioluminescence Tumor Monitoring in Comparison with...
29 June 2024 9,856 1 View
Hi , in my study I will compare the effectiveness of Al generated feedback and traditional feedback in writing lesson.It will be a quasi-experimental design,but l have 3 classes not two.What...
25 June 2024 6,244 3 View
If we find sulfides in subduction zone interface rocks, how can we distinguish whether these sulfides are related to crust-derived fluids or originate from the original mantle wedge? Especially...
15 June 2024 654 1 View
Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...
05 June 2024 624 2 View
Hi, I am doing experiment of lignin deposition on tomato leaves after fungi infection by phloroglucinol-HCl staining. But the problem here is that the leaves remain yellowish or blackish before...
08 May 2024 2,127 0 View
I need to write a 5000 word, literature review and I have chosen an integrative review using Whittemore & knafl. I'm struggling to decide how I should best set this out, which topics to use in...
30 April 2024 365 0 View
Hi Research Gate people! I am trying to determine the sample size, power and alpha boundary needed for my interim analysis for a registered report in a psych journal. I have read the paper by...
28 April 2024 3,444 2 View
X=categorical IV (X1, X2) M1,M2,Y=continuous variables First: 1. compare X1 and X2, and hypothesize that X1 will predict higher Y 2. X1 will predict M1 3. M1 will predict M2 4. X1 will predict...
26 April 2024 6,207 0 View
I am doing a replication of an experiment regarding attractiveness and I am looking for photos I could use in it.
22 April 2024 5,297 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,827 0 View
Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...
01 August 2024 4,456 2 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
In the aquaculture and fish nutrition research field, a number of growth and somatic indexes are often used, including specific growth rate (SGR), feed conversion rate (FCR), protein efficiency...
22 July 2024 2,479 4 View